Intermediate

Part:BBa_J31015:Design

Designed by: Erin Zwack   Group: iGEM06_Davidson   (2007-12-17)


crRNA-GVPLVA-tt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 688


Design Notes

The part cannot be cut with Xba unless grown in dam/- cells


Source

crRNA and GVPLVA-tt parts were ligated together

References